Morphoseq™ – a shorter way to longer reads
We have developed Morphoseq technology to convert short read sequencers into ‘virtual long read’ sequencers, enabling finished quality genome assemblies with high accuracy, including resolution of difficult-to-assemble genomic regions.
Morphoseq is designed to dramatically improve the performance of third party NGS platforms, especially ‘short read’ systems.
With Morphoseq, long read capabilities can be harnessed with the cost efficiency, accuracy and wide availability of short read sequencers.
How does it work?
Our technology utilises a proprietary mutagenesis reaction to introduce unique mutation patterns into long DNA molecules, up to 10 kbp and greater. This creates a unique “identifier” for each molecule in the sample. The samples are then pooled (“multiplexed”) and amplified via a limited cycle, low bias PCR to make many copies. Finally, these are fragmented and sequenced on existing short read platforms.
Our custom Morphoseq algorithm uses the unique identifiers to reconstruct the original long DNA template sequences. The resulting long DNA sequences are extremely high quality, with typical accuracy in excess of 99.9% (> Q30). This enables the assembly of contiguous genomes de novo and resolution of difficult to assemble genomic regions which are of biological and clinical significance.
High quality resolution of bacterial genomes
When applied to bacterial genomes, Morphoseq can produce completely closed chromosomes in a single scaffold.
The diagram below shows short read (left) vs Morphoseq (right) assembly graphs for bacteria species with a range of GC contents.
Each assembly contig (a contiguous regions of DNA) is depicted as a curved line segment. When repeat sequences prevent the assembler from resolving contig adjacencies with short reads alone, the ends of the possibly adjacent contigs are joined to each other in the diagram.